Plant & Animal Genome IX Conference

Workshop: Forage & Turfgrass
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EST DISCOVERY AND MICRARRAY-BASED GENE EXPRESSION PROFILING IN WHITE CLOVER (TRIFOLIUM REPENS)

Plant Biotechnology Centre, Agriculture Victoria, La Trobe University, Bundoora, Victoria 3083, Australia

White clover (Trifolium repens) is a major forage legume of temperate pastures in many regions around the world. An expressed sequence tag (EST) discovery program has been undertaken using high-throughput sequencing of randomly selected clones from cDNA libraries representing a range of white clover plant organs, developmental stages, and environmental treatments. Over 40,000 ESTs have been generated, analysed by BLAST searches and categorised functionally. Analysis of gene expression using high density cDNA microarrays has been performed as a main screening tool for novel sequences of unknown function. Comparative sequence and microarray data analysis from white clover and Medicago truncatula ESTs will provide insight into conserved and divergent aspects of legume genome organization and function.