Plant & Animal Genome IX Conference

Workshop: Cotton
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INTEGRATED GENETIC AND PHYSICAL MAPPING IN COTTON

New Mexico University, PO Box 30003, Las Cruces, NM 88003-8003 USA

Progress towards an integrated map is feasible in cotton using all available genetic and DNA marker resources. Our focus has been on portable PCR-based DNA markers, primarily DNA microsatellites or simple sequence repeats (SSRs). The chromosomal and genetic map location of many of the SSR loci will be discussed. SSRs are being anchored to physical mapping resource utilizing a large-insert Maxxa BAC library. BAC-PCR pools provide an efficient way for localizing an SSR loci to a BAC address, thus converting the SSR to a sequence tagged site. These provide framework markers for future genetic and physical mapping efforts. The analysis of the BAC library with the SSR markers can also provide valuable information on the quality of the BAC resource.