Plant & Animal Genome IX Conference

Workshop: Brassicas
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APPLICATION OF BAC LIBRARY FOR CHINESE CABBAGE GENOME RESEARCH

¹ Chungnam National University, Taejon, 305-764, Korea

² Nat'l Inst. of Agricultural Science & Technology, 249 Seodundong, Suwon 441-707, Korea

³ Division of Biology 147-75, California Institute of Technology, Pasadena, CA 91125, USA

The genus Brassica includes three diploid species which have respective A, B, and C genomes, is the most closely related group of crop to Arabidopsis, and embodies numerous important crop species grown as oilseeds, vegetables, fodder and source of condiments in Asia, USA, and Canada. Especially Chinese cabbage, A genome, is one of the most widely used crops as dried, pickled, or cooked vegetable in northeast Asian region. Bacterial artificial chromosome (BAC) system is commonly used tool for the genomic research. In the present study, a Chinese cabbage inbred line and BAC vector system have been adapted for constructing Brassica A genomic library. As the genome size of Chinese cabbage is 550 Mbp, the HindIII BAC library consisting 56,592 clones which contain 115 kb average insert size is estimated for 11-fold coverage of the whole genome. As the result of Southern analysis, we confirmed that BAC clones of library have Chinese cabbage genome DNA as insert. We also confirm that about 4% of BAC library was contaminated with chloroplast genome. For the application of BAC library for Chinese cabbage genome research, the generating of the physical contig map, and BAC-end sequencing data will be presented. Also, we tried the comparative genetic mapping among Arabidopsis, Brassica A and C genome, using 14 predicted genes from Arabidopsis. Finally, we set up the fluorescent in situ hybridization (FISH) method using BAC clones, to confirm the large genomic DNA localization on plant chromosomes.