Plant & Animal Genome IX Conference

Workshop: Array Technology
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CDNA MICROARRAY SYSTEM FOR THE ANALYSIS OF RICE GENE EXPRESSION II: MICROARRAY WITH 9000 NON-REDUNDANT EST CLONES AS PROBE

¹ Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries

² National Institute of Agrobiological Resources

³ Department of Science, Kyushu University

As reported in PAGVIII (2000), using 1265 non-redanant EST clones from rice as probe, we have constructed the full-insert and 3'-UTR cDNA microarray system for the pilot-scale rice gene expression analysis. 3'-UTR array system shows better gene-specific expression data than the full-insert array system. After that, number of probe cDNAs were increased and the non-redundant 9000 full-insert microarray system has been established. Information of the probe cDNA clones will be published on the WEB. According to the genome DNA size of rice, number of the expressing gene in rice is estimated to 20,000 to 40,000. About 9000 EST clones seem to cover only the part of the genome. In order to obtain the uncovered genes and for the genome DNA coding region analysis and the information of protein data, the rice full-length cDNA project has been also launched since the beginning of Year 2000. As a prospect, the data of the full-length cDNA project will be transferred to the microarray system. This microarray system has been established by the project budget of rice genome project in Japan. Target RNAs are prepared and supplied from about 60 project research groups. Research thema of the project are composed of several stress responses and the tissue and organ specific expression of rice and other plants. Data of the 9000-array system are just started to be obtained, however, new and interesting genes will be propectively cloned by this system.