Plant & Animal Genome IX Conference

Workshop: Aquaculture
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AN UPDATED LINKAGE MAP OF RAINBOW TROUT (Oncorhynchus mykiss) USING DOUBLED HAPLOIDS

¹ School of Biological Sciences, Washington State University, Pullman, WA 99164-4236

² Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640

³ Department of Zoology, University of Guelph, Guelph, ON Canada N1G 2W1

⁴ Department of Biological Sciences, University of Wisconsin - Milwaukee, PO Box 413, Milwaukee, WI 53201

⁵ University of Washington, School of Fisheries, Seattle, WA 98105

Genome mapping in salmonids has important implications for comparative mapping and provides the framework for study of the underlying genetics of physiological, morphological, and behavioral traits. Our initial rainbow trout linkage map (Young et al 1998) was produced using 76 androgenetic doubled haploid individuals produced from a hybrid of the Oregon State University (OSU) and Arlee (ARL) clonal rainbow trout lines. This linkage map consists of AFLP, RFLP, RAPD, and a few microsatellite markers. Previously, the 476 markers segregated into 31 major linkage groups and 11 small groups. We report a significant update of the rainbow trout linkage map. The doubled haploid population has proven advantageous in marker scoring and mapping, especially of Type I markers. The collaboration of multiple laboratories has resulted in the addition of approximately 700 AFLP, 100 microsatellites, and 20 Type I loci to the map. A subset of 50 individuals from the original mapping population were genotyped for these new markers. The update of the linkage map is ongoing, with an emphasis on adding known genes and other non-AFLP markers to the map. The addition of 100 microsatellite markers also found on the recently published O. mykiss maps from SALMAP (Sakamoto et al., 2000) will allow us to provide synteny among these two rainbow trout maps. The increased resolution of this map should allow fine scale analysis of evolutionary and economically important traits.