Plant & Animal Genome IX Conference

Workshop: Aquaculture
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MAPPING QUANTITATIVE TRAIT LOCI FOR PRODUCTION TRAITS OF THE KURUMA PRAWN PENAEUS JAPONICUS USING AFLP MARKERS

¹ CSIRO Livestock Industries, Molecular Animal Genetics Centre, Level 3, Gehrmann Laboratories, University of Queensland, St. Lucia 4072, Queensland, Australia

² Dipartimento di Biologia, Università di Roma

³ Dept. of Agriculture, Food and Nutritional Science, University of Alberta, 4-10 Ag/For Building, Edmonton T6G 2P5, Canada

⁴ CSIRO Marine Research, Cleveland 4163, Queensland, Australia

A full-sib family of Penaeus Japonicus was developed for the identification of growth QTL. Phenotypic data for growth traits have been obtained from over 400 progeny. Two-stage selective genetic mapping strategy was applied in the study. An initial mapping of 46 extreme progeny from two tails using 54 pairs of AFLP primer combinations had resulted in nearly 70% genome coverage. The preliminary QTL analysis indicated the existence of potential QTL for the growth traits. In order to verify the results, a second stage of linkage mapping on the additional 56 progeny was conducted. A total of 401 markers following the Mendelian segregation ratio of 1:1 were generated from the population of 102 progeny. Forty-three linkage groups were obtained for male map and thirty-one for female map, in comparison to the haploid number of 44 chromosomes of P. japonicus. The final QTL analyses from combined data identified two chromosome regions that had significant effects on the growth traits.