Plant & Animal Genome IX Conference

Poster: Aquaculture
P5o_01.html

A SECOND GENERATION LINKAGE MAP OF TILAPIA (OREOCHROMIS SPECIES)

¹ Department of Zoology and Program in Genetics, University of New Hampshire, Durham, New Hampshire, 03824, USA

² GenoMar As, Oslo Research Park, Gaustadalleen 21, N-0349, Norway

³ Department of Aquaculture, Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel

We have constructed detailed genetic linkage map for tilapia, based on an interspecific F2 family (Oreochromis. aureus X O. niloticus). Five hundred and five microsatellite markers including 5 genes (IGF2, Insulin, RAS-GRF2, UV opsin, blue opsin) have been used for this linkage map. Four hundred eighty one (95%) of these markers segregated into 22 major linkage groups and 3 small groups (<6 markers/group) that may indicate chromosomal translocations between O. aureus and O. niloticus. The genome size is estimated to be 2184.5 cM in length which is nearly twice long as the previous estimate based on haploid larvae. Average spacing between markers is 4.3 cM and number of markers per each linkage group varies from 4 to 55. Additional markers are being genotyped to increase the resolution of this map. (http://tilapia.unh.edu)