Plant & Animal Genome IX Conference

Poster: Wheat, Barley, Rye, Oat
P5c_16.html

IDENTIFICATION AND USE OF TENTATIVE UNIQUE ESTS FOR GENE MAPPING WITH DELETION STOCKS IN WHEAT.

¹ University of California, Davis. USDA-ARS, WRRC, 800 Buchanan St., Albany, CA 94710 USA

² USDA-ARS, WRRC, 800 Buchanan St., Albany, CA 94710 USA

The availability of chromosomal deletion stocks for hexaploid wheat provides a unique system for the physical mapping of genes. The National Science Foundation Wheat Genome Project has the objective of mapping 10,000 singleton expressed sequence tags (ESTs) to chromosomal bin locations by utilization of deletion stocks. A selected panel of 94 deletion lines (supplemented by 14 ditelosomic lines) provides coverage of the wheat genome to an average bin size of 10 cM. We present here a methodology for selecting tentative unique ESTs from a large pool of wheat cDNA sequences. The system includes assembly of 5' sequence data to identify initial redundancies, comparison of candidates to published EST sequences, and 3' sequencing to filter remaining duplications. Our group contributes to this physical mapping goal through the generation of ESTs, their screening for redundancy and repetitive genetic elements, and the preparation of PCR products that may be hybridized to Southern blots of deletion line DNA. Preliminary data from the members of the project who probe the deletion mapping blots has shown that this method provides an easily scorable system for mapping ESTs in wheat.