1 1 Shields Ave, Department of Animal Science, Meyer Hall, UCDavis, Davis, CA 95616 USA 2 Clear Springs Food, Inc., Research Division, P.O. Box 712, Buhl, ID 83316 USA
Infectious hematopoietic necrosis (IHN), caused by a rhabdovirus, is among the diseases that constrain fish production in the United States, especially of important species like salmonids as trout and salmon. A study is being conducted to locate molecular marker(s) linked to quantitative trait loci (QTLs) confering resistance to IHN in rainbow trout. F1 families were produced by crossing rainbow trout and Oxbow steelhead trout. Males of each family (F1) were backcrossed with rainbow trout producing 70 mapping families. The progeny were challenged with the IHN virus. The crosses and challenges took place at Clearsprings (ID). Initially one family segregating for the trait in question is being screened for amplified fragment length polymorphisms (AFLP), at the Genome Variation Lab in UCDavis. Sixty-four primer combinations are being used with the expectative of obtaining about 300 markers. The linkage data are being analyzed with the software package Mapmaker. At the conclusion of the experiment, a breeding plan to increase IHN resistance in rainbow trout will be developed.
Plant & Animal Genome VII Conference Plant & Animal Genome VII Conference