EVALUATION OF THREE OREOCHROMIS NILOTICUS STRAINS IN THE PROCESS OF CONFORMATION OF TILAPIA GENOME BANK

¹ Departamento de Ingeniería de Proyectos CUCEI, Universidad de Guadalajara, PO Box 307, Zapopan, Jal. México
² CEUNIVO, Universidad de Colima, PO Box 565, Manzanillo, Col.28200, México
³ Centro Universitario de Ciencias Biologicas y Agropecuarias, Universidad de Guadalajara

The main goal of this research is the conformation of a genome bank of tilapia (Oreochromis) and the respective studies that guarantee the introduction of certain varieties of Oreochromis niloticus, the most suitable species from the aquacultural productivity point of view. Therefore, studies about productivity, genetics and immunology were realized with the objective to evaluate three varieties Of O. niloticus (O. n. var. Egyptian, var. Stirling and var. Rocky moutain).
Growth, proliferative reproduction and fillet yield of the three varieties were compared in the productive field research.
Parallel genetic and immulogical studies were also performed to determine the interrelation degree and the immune response between these varieties of Oreochromis.
The cellular immune response was realized with a blastoid transformation assay in lymphocytes utilizing a constructed dose-response curve with the mitogene phytohemaglultinine (PHA); lymphocytic cultures of the three Oreochromis strains were compared among them.
We applied the RAPDs technique to evaluate the genetic relationship, as well as to assess their purity degree in order to have genetic background to start establishing the genomic bank.