PAG-V: W93 - GENETIC LINKAGE MAPS OF SALMONID FISHES: SALMAP

Plant & Animal Genome V Conference

Town & Country Hotel, San Diego, CA, January 12-16, 1997.

W93

GENETIC LINKAGE MAPS OF SALMONID FISHES: SALMAP

Lie, Oystein(1), R. Danzmann(2), R. Guyomard(3), L.E. Holm(4), R. Powell(5), A. Slettan(6), J. Taggart(7)
1. Division of Genetics, Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine,P.O.Box 8146, Dep. N-0033, Oslo, Norway
2. University of Guelph, Canada
3. INRA, Jouy-en-Josas, France
4. Danish Institute of Animal Science, Viborg, Denmark
5. University College Galway, Galway, Ireland
6. Norwegian College of Veterinary Medicine, P.O.Box 8146. Dep. N-0033, Oslo, Norway
7. University of Stirling, Stirling, Scotland

SALMAP is a collaborative project between five European and one Canadian laboratory, funded by the European Community. The objective of the SALMAP project is to further develop the salmonid maps by producing and characterizing highly informative DNA markers with particular stress on microsatellites in Atlantic salmon (Salmo salar), rainbow trout (Onchorhyncus mykiss) and brown trout (Salmo trutta). Previous research has led to a composite salmonid linkage map by analysis based on type I markers (e.g. ~ 50 allozyme loci) in intra- and inter-specific crosses of salmonid species, including gynogenetic crosses. Current research is assigning simple sequence length polymorphisms to linkage groups in several salmonid species. Moreover, microsatellite linkage groups have recently been anchored with existing allozyme loci and cosegregation of markers with potential QTLs (upper temperature tolerance) have been revealed in rainbow trout. SALMAP collaborators will type the informative markers in reference families, some of which are also typed for allozymes. Based on the linkage analysis data, low resolution genetic maps of the three species will be constructed, embracing mostly anonymous markers but also enzyme loci. The potentials of ploidy techniques in fish genome analysis will be exploited e.g: haploid gynogenesis in linkage mapping, meiotic diploid gynogenesis in linkage orientation and centromere mapping, and mitotic diploid gynogenesis together with classical Mendelian families in QTL mapping. Depending on the success rate of cross reacting markers and the degree of interspecific conservation of marker linkage groups, anchor loci in a comparative salmonid map will be identified.