Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W11 - A GENETIC LINKAGE MAP OF THE TILAPIA (Oreochromis niloticus)
W11
A GENETIC LINKAGE MAP OF THE TILAPIA (Oreochromis niloticus)
KOCHER, THOMAS D.(1), Woo-Jai Lee(1), Halina Sobolewska(2), David Penman(2), Brendan McAndrew(2)
1. Department of Zoology, University of New Hampshire, Durham, NH 03824
2. Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK
We have constructed a genetic map for tilapia (Oreochromis niloticus) using DNA markers. The segregation of 62 microsatellite and 112 AFLP (total = 174) polymorphisms was studied in 41 haploid embryos derived from a single female. We have identified linkages among 152 (92.1%) of these markers. 97% of the microsatellites and 89% of the AFLP polymorphisms were linked in the final map. The map spans 791.5 Kosambi centimorgans in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart we estimate a total map length of approximately 1,000 to 1,200cM. The data suggest that the microsatellite markers are slightly clustered in the genome. This map is a starting point for the mapping of quantitative traits in cichlid fishes.WWW-site for the tilapia map.