Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: P232 - AFLP AS A TOOL TO FIND MOLECULAR MARKERS LINKED TO NEMATODE RESISTANCE GENES IN <i>A. hypogaea</i> L.
P232
AFLP AS A TOOL TO FIND MOLECULAR MARKERS LINKED TO NEMATODE RESISTANCE GENES IN A. hypogaea L.
LOPES, CATALINA ROMERO(1), Marcos Aparecido Gimenes(1), Gary Kochert(2), Harold Thomas Stalker(3), Gustavo Garcia(3)
1. Depto. de Genetica-IB-Universidade Estadual Paulista (UNESP)- Botucatu-SP-Brazil
2. Dept. of Botany-University of Georgia-Athens-GA-USA
3. Crop sciences Department, North Carolina State University, Raleigh, NC, 27965, USA
Arachis hypogaea, the cultivated peanut, has a limited genetic pool and gene introgression from related wild species is an important breeding tool. AFLP analysis and a DNA pooling strategy were used to find markers linked to nematode resistance. A number of introgression lines derived from a cross between A. hypogaea and A. cardenasii were screened for nematode resistance, and line GA6 was found to be highly resistant. An F2 population segregating for nematode resistance was then produced by crossing GA6 with A. hypogaea. DNA pools were formed by selecting the most resistant and the most susceptible F2 plants. The resistant and susceptible pools were compared with A. hypogaea, GA6 and A. cardenasii by AFLP analysis.One AFLP fragment was found to be present in the resistant pool, in A. cardenasii and in GA6, but absent from A. hypogaea and the susceptible pool. This fragment was cloned and sequenced. Primers were designed and PCR was performed. The fragment was not amplified in the plants making up the susceptible pool, but was amplified in the resistant plants suggesting the marker is linked to the A. cardenasii gene that confers resistance to nematodes.