PAG-V: P185 - RAPD DNA MARKERS LINKED TO A GENE IN WHEAT FOR RESISTANCE TO SEPTORIA TRITICI BLOTCH

Plant & Animal Genome V Conference

Town & Country Hotel, San Diego, CA, January 12-16, 1997.

P185

RAPD DNA MARKERS LINKED TO A GENE IN WHEAT FOR RESISTANCE TO SEPTORIA TRITICI BLOTCH

YANG, XIAOKUN(1), Herbert W. Ohm(1), Ismail Dweikat(1), Gregory Shaner(2)
1. Department of Agronomy, Purdue University, 1150 Lilly Hall, West Lafayette, IN 47907-1150
2. Department of Botany and Plant Pathology, Purdue University, Lilly Hall, West Lafayette, IN 47907-1150

Resistance to Indiana isolates of Septoria tritici Roberge in Desmaz. in the Chinese wheat (Triticum aestivum L.) line SO852 is conditioned by a single dominant gene. One hundred and thirty-eight F_2:9 recombinant inbred lines were developed by single seed descent from a cross between SO852 and susceptible winter wheat cultivar Clark. A pair of near isogenic lines from a single F₈ plant that was segregating for Septoria tritici blotch (STB) resistance were identified. The two lines, 881072-1 (resistant) and 881072-2 (susceptible), were used to screen for RAPD polymorphisms. Two RAPD markers, OPG7₁₁₀₀ and OPH19₅₂₀, were identified as co-segregating with resistance in a set of 48 individual F_2:9 recombinant inbred lines. OPH19₅₂₀ also is present in 10 other STB resistant wheat germplasm lines, including Kavkaz/K4500 1.6.a.4, Bulgaria 88, and Veranopolis, but not in Israel 493. Linkage analysis and chromosomal location of the STB resistance gene will be presented.