Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: P144 - GENETIC ANALYSIS ON THE RESISTANCE TO GREEN RICE LEAFHOPPER IN A <i>JAPONICA</i> IMPROVED LINE, NORIN PL6, USING MOLECULAR MARKERS.
P144
GENETIC ANALYSIS ON THE RESISTANCE TO GREEN RICE LEAFHOPPER IN A JAPONICA IMPROVED LINE, NORIN PL6, USING MOLECULAR MARKERS.
TAMURA, KATSUNORI, Masahiro Hirae, Shingo Oya, Ikuo Ashikawa, Tadashi Yagi, Yoshimichi Fukuta
Hokuriku National Agricultural Experiment Station, MAFF., Inada 1-2-1 Joetsu Niigata 943-01. JAPAN
We have determined the chromosomal locations of two dominant genes, which act complementarily for resistance to greeen rice leafhopper in Norin PL6, using RFLP markers. The resistant genes of Norin PL6 had been introgressed from an indica variety, Lepedumai, to a japonica variety, Toyonishiki. As a first step toward identifying the loci of the resistant genes, we performed RFLP analysis of Lepedumai, Norin PL6 and Toyonishiki using 120 markers and represented the genotype of Norin PL6 in a graphical form. Two introgressed segments originating from Lepedumai were detected on chromosome 3 and chromosome 11 in Norin PL6, which suggests that these two chromosomal regions contain the resistant genes to green rice leafhopper. As a second step, we conducted linkage analysis between the resistant genes and detected RFLPs using a B1F1 (Toyonishiki/Norin PL6//Toyonishiki) population. The B1F1 plants segregated into 1:3 for resistant and susceptible, indicating that the resistance of Norin PL6 was controlled by two complementary genes. We identified the linkages between these genes with RFLP markers on chromosome 3 and RFLP markers on chromosome 11,respectively.