Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: P12 - KNOB REPEATED SEQUENCES ISOLATED FROM A MAIZE CHROMOSOME 9 ADDITION LINE OF OAT
P12
KNOB REPEATED SEQUENCES ISOLATED FROM A MAIZE CHROMOSOME 9 ADDITION LINE OF OAT
ANANIEV, EVGUENI V.(1), Oscar Riera-Lizarazu(1), Ronald L. Phillips(2), Howard W Rines(2)
1. Department of Agriculture and Plant Genetics, University of Minnesota, 1991 Buford Circle, 503 Borlaug Hall
2. Department of Agriculture and Plant Genetics, University of Minnesota, 1991 Buford Circle, 513 Borlaug Hall
Maize (Zea mays L.) chromosome addition lines of oat (Avena sativa L.) recovered from oat x maize crosses serve as a source of DNA to construct cosmid libraries and isolate maize chromosome-specific clones. The maize DNA-containing clones can be selectively isolated from the library with the help of a multiprobe - a mixture of maize-specific highly repetitive dispersed sequences. In this research we demonstrate the potentials of this system for the isolation of DNA fragments of clustered repeated sequences from a specific maize chromosome. We isolated a group of 50 cosmid clones with knob-repeated sequences from maize chromosome 9 by screening a maize chromosome 9 addition line library using the maize 185 bp knob sequence repeat. The recovered clones, about 1.5 Mb of knob DNA sequences, may be classified into several groups on the basis of restriction maps and nucleotide sequence composition. We found that knob sequences form stretches of tandem repeats interspersed with other types of nucleotide sequences. Some of them are highly repeated dispersed elements found in other maize chromosomes and some of them are low copy number sequences. A certain level of heterogeneity was observed between copies of knob repeated sequences that originated presumably from one knob on chromosome 9.