PG-II: Saccharum spontaneum Genetic Linkage Map Containing PCR and RFLP Markers

Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.

Saccharum spontaneum Genetic Linkage Map Containing PCR and RFLP Markers

Jorge da Silva 2,3, Salah M. Al-Janabi 1, William Burnquist 3, Rhonda J. Honeycutt 1, Mark E. Sorrells 2, Steven D. Tanksley 2, and Bruno W.S. Sobral 1. 1 California Institute of Biological Research, 11099 N. Torrey Pines Rd., Suite 300, La Jolla USA 92037, 2 Department of Plant Breeding and Biometry, Cornell University, Ithaca NY USA 14853, and 3 Copersucar Sugarcane Technology Center, 13.400 Piracicaba, SZio Paulo, Brazil

A 527-marker linkage map of Saccharum spontaneum L. 'SES 208' (2n = 64) was established by uniting 208 single-dose arbitrarily primed PCR polymorphisms, 234 single-dose RFLPS, 41 double-dose (DD) and 1 triple-dose (TD) polymorphisms. A map hypothesis constructed using these markers (minimum L.O.D. = 4.00, theta = 0.20 M) had 64 linkage groups that fell into 8 homology groups, suggesting that x = 8 in this species. Homology groups were identified by using DD and TD fragments as well as highly polymorphic single-dose RFLPs that identified more than one linkage group. Linkages in repulsion were absent from the map, as found in two previous studies of this species. Together, these data demonstrate that SES 208 displays random chromosome assortment, a behavior typical of autopolyploid species. As with previous studies, it was concluded that SES 208 behaves like an auto-octaploid species. A X^2 was used to test whether the 527 markers were randomly distributed throughout the genome: both arbitrarily primed PCR and RFLPs had a distribution that was statistically equal to random. The integrated arbitrarily primed PCR-RFLP map had a predicted genomic coverage of 93% (using only single dose polymorphisms) and an average interval between markers of 6 cM. Furthermore, markers were used to estimate the genome size of SES 208, which was approximately 3,300 cM.

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