Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: MAPPING OF THE COCOA GENOME USING BIOCHEMICAL AND MOLECULAR
MARKERS
MAPPING OF THE COCOA GENOME USING BIOCHEMICAL AND MOLECULAR
MARKERS
C. Lanaud, A.M. Risterrucci, J.A.K. N'goran and D. Clement,
CIRAD-BIOTROP and CIRAD-CP, BP 5035, 34032 Montpellier Cedex,
France.
A map of the cocoa genome is being constructed using
biochemical and molecular markers. A genomic library, and a cDNA
library established using germinated beans from the clone IFC5
(Amelonado) were constructed. After extraction techniques had
been optimized, the selection of polymorphic probes from these
libraries was made using a sample of six clones belonging to
different genetic groups and, using 5 restriction enzymes, 45% of
the probe were shown to be polymorphic for at least one
restriction enzyme. The selection and segregation of RAPD
type markers were also studied; the primers used are from the
OPERON kits. By using populations of the back-cross type for
this mapping it was possible to construct a first map integrating
close to one hundred RFLP, RAPD and isozyme markers.
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