PAG-II Plant Genome II Conference

Town & Country Conference Center, San Diego, CA, January, 1994.


PG-II: THE RFLP LINKAGE MAP OF THE POTATO AND ITS USE FOR MAPPING QUALITATIVE AND QUANTITATIVE TRAITS

THE RFLP LINKAGE MAP OF THE POTATO AND ITS USE FOR MAPPING QUALITATIVE AND QUANTITATIVE TRAITS.

Christiane Gebhardt, Enrique Ritter, Christiane Leonards-Schippers, Ralf Schafer-Pregl, Werner Gieffers, Amalia Barone, Ali El-Kharbotly, Jens Niewohner and Francesco Salamini, MPI fur Zuchtungsforschung, Carl-von-Linne-Weg 10, 50829 Koln, Germany.


An RFLP linkage map has been constructed based on an intraspecific cross between two partially heterozygous, diploid potato lines. The map consists of more than 400 RFLP loci and covers ca. 90% of the potato genome. The genomic positions of a series of functional genes of the potato have also been included in the map. The RFLP linkage map was used to locate in the potato genome monogenic resistance traits to the root cyst nematode Globodera rostochiensis, potato virus X and the fungus Phytophthora infestans. Quantitative trait loci (QTL) for resistance to P. infestans have also been mapped. RFLP markers closely linked to nematode resistance loci were converted into allele specific PCR primers to be used in marker based selection schemes.


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