Plant & Animal Genomes XIV Conference Plant & Animal Genomes XIV ConferenceJanuary 14-18, 2006
Town & Country Convention Center
San Diego, CA
Isabel A. del Blanco , Deric A. Schmierer , Steven E. Ullrich , Andris Kleinhofs
The production of cultivars having both high malting quality and grain yield is a major goal for barley breeders. The high yielding, two-row, feed cultivar Baronesse is the leading barley grown in Washington State. The transfer of yield QTL from Baronesse to the two-row malting standard Harrington will translate in the creation of new cultivars with high yield and Harrington-like malting characteristics. Putative Baronesse grain yield QTL have been identified on chromosomes 2HL (two regions, flanked by markers ABG461C-MWG699 and ABG072-ksuD22) and 3HL (MWG571A-MWG961). A near isogenic line (NIL), 00-170, generated from crosses between Harrington and Baronesse, does not yield consistently equal to Baronesse, but does maintain the malting quality of Harrington. NIL 00-170 has the Baronesse QTL on 3HL. Crosses were made between 00-170 and other NIL carrying QTL for grain yield on different genomic regions, and advanced to F2. Samples from 715 F2 lines were collected from the field and genotyped for putative Baronesse grain yield QTLs. Cleaved amplified polymorphic sequences (CAPS) markers and specific primers exploiting single nucleotide polymorphisms (SNP) targeting these genome regions were developed and used for genotype selection. Selection of 219 F2 plants was made on the basis of genotype screening using CAPS marker MWG571A/Bam HI. Further selection using CAPS MWG699/Taq1 and SNP reduced the selected samples to 51. Selected lines having these QTLs will be increased in the greenhouse and tested in the field. The evaluation of lines having one, two, and three yield QTLs, and their different combinations will allow the identification of higher yielding elite lines and the estimation of the QTL effects on grain yield.