Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

NEW CHICKEN, TURKEY, SALMON, BOVINE, PORCINE AND SHEEP GENOMIC BAC LIBRARIES TO COMPLEMENT WORLD WIDE EFFORT TO MAP FARM ANIMALS GENOMES

¹ BACPAC Resources, Children’s Hospital Oakland Research Institute, 747 - 52nd St, Oakland, CA 94609

² Norwegian School of Veterinary Science, Oslo, Norway

To facilitate the production of farm animals with improved production traits and to possibly help in finding cures for human diseases we recently created 10-15 fold redundant bacterial artificial chromosome (BAC) libraries using EcoRI-partially digested DNA from Chicken – CHORI-261, Turkey - CHORI-260, Atlantic Salmon - CHORI-214, Holstein bull - RPCI-42 and the Hereford bull - CHORI-240 (constructed using MboI partially digested genomic DNA), Pig - CHORI-242 and Sheep - CHORI-243 with the average insert sizes for these libraries: 195Kb, 192Kb, 188Kb, 164Kb, 167Kb and 173Kb respectively (sheep library has not been characterized yet) with a low percentage (<5%) of non-recombinant clones. Constructed BAC libraries will play an essential role for genome mapping and sequencing of these animals. All libraries are publicly available and additional information can be obtained through our web site: http://www.chori.org/bacpac This work was funded mostly as part of the USDA-ARS