Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

AN EFFICIENT METHOD FOR THE CONSTRUCTION OF INTEGRATED GENETIC AND PHYSICAL MAPS : AFLP FINGERPRINTING OF HIGH QUALITY BAC LIBRARIES AND BAC POOLS ON THE MEGABACE 1000 CAPILLARY SEQUENCER

¹ Keygene N.V., PO Box 216, 6700AE, Wageningen, the Netherlands

² Max-Planck Institute for Developmental Biology, Spemannstrasse 35, D-72076, Tuebingen, Germany

The availablity of high insert size genomic libraries and integrated genetic and physical maps is an indispensable tool in genomic research. One remaining challenge in integrated map construction is the efficient linkage of the BAC contigs to the genetic map. A high throughput platform for the detection of AFLP fingerprints of individual or pooled BACs was developed. All AFLP fingerprints are analysed by capillary electrophoresis on the MegaBACE 1000 capillary sequencer. Traces of each individual BAC are processed by FPC and inhouse software. The integration of the data generated by individual AFLP BAC fingerprinting and the subsequent screening of the BAC pools with mapped AFLPs is fully automated. One major advantage of the screening of individual BACs with AFLP is the faster merging of the BAC contigs with genetic AFLP maps when compared to the classic fingerprinting of BACs on agarose gels. Consequently the integrated maps constructed with AFLP fingerprinting of BACs are more accurate and less time consuming to construct than those based on the agarose gel based approach. We are constructing a high resolution integrated genetic and physical map of Pristionchus pacificus for the Max-Planck Institute in Tuebingen and the construction of integrated genetic and physical maps of larger plant genomes has recently started. AFLP is a registered trademark of Keygene N.V. For more information please visit our website http://www.keygene-genomics.com/ or send your email to taco.jesse@keygene.com