PAG-XI  Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA


Poster: Genome Sequencing & ESTs
            


P60

IDENTIFYING EXPRESSED GENES FROM ADULT GLASSY-WINGED SHARPSHOOTERS, HOMALODISCA COAGULATA (SAY), (HEMIPTERA: CICADELLIDAE).

WAYNE B. HUNTER1 , MICHAEL G. BAUSHER1 , PHAT DANG1 , HEATHER COSTA2 , JOSE X. CHAPARRO1 , ROBERT G. SHATTERS, JR.1 , CINDY L. MCKENZIE1 , XIOMARA H. SINISTERRA1

1 USDA,ARS,U.S. HORTICULTURAL RESEARCH LAB, 2001 SOUTH ROCK, RD., FT. PIERCE, FL 34945, USA
2 UNIVERSITY OF CALIFORNIA, ENTOMOLOGY DEPT., RIVERSIDE, CA, 92521, USA

The severe impact of Pierce’s Disease upon the U.S. grape industry has generated the need to rapidly understand the epidemiology of this disease. The primary insect vector is the the glassy-winged sharpshooter leafhopper, GWSS. Our objectives were to develop expressed sequence tags to increase our understanding of leafhopper biology. The genetic information will then be used to develop environmentally friendly, sound management programs to reduce the spread of Pierce’s Disease. Producing cDNA libraries of the GWSS allowed examination of genes being expressed within adult GWSS feeding on citrus. Over 9,000 sequences were generated, from adult GWSS collected from citrus in a California grove. The genes sequences forming the top 10 contigs and their putative functions are listed. Information on the library cDNA population dynamics is provided.


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