CHINESE CABBAGE BAC END SEQUENCE ANALYSIS AND COMPARISON WITH Arabidopsis

Department of Horticulture, and Genome Research Center, Chungnam National University, Daejeon, 305-764, Korea

BAC end sequence(BES) provide the high specific sequence markers in the large-scale genome sequencing project. BES is very useful in sequence assembly using STC (sequence tagged connector) framework and to search for protein coding region, transposable elements, EST contents, and STS (sequence tagged site) marker development. Chinese cabbage (Brassica rapa L. spp pekinensis) 1,659 BESs were analyzed, and an average of reads size of those were evaluated 327bp based on Phred quality value (sequence quality value) 20. Chinese cabbage BES contained approximately 23.1% protein coding regions, 20.1% transposable elements, 2.5% EST contents. the primers for STS marker were designed using 161 protein coding region sequence in BES, and the polymorphisms for those PCR products have been identifying to use as genetic markers. And we have attempted the comparison Arabidopsis between Chinese cabbage using Chinese cabbage BES. So we intend to deduce for the gene distribution in Chinese cabbage genome.