Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Workshop: Aquaculture
            

TOWARD THE POSITIONAL CLONING OF THE DOMINANT ALBINO GENE OF RAINBOW TROUT

¹ Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4, Minato-ku, Tokyo 108-8477,Japan

² Fuji Trout Hachery, Shizuoka Prefecture Fisheries Experimental Station, Fujinomiya, Shizuoka 418-0108 Japan

Oculocutaneous albinism (OCA) is an inherited disorder. Although OCA is a recessive trait in general, an autosomal dominant OCA strain of rainbow trout has been isolated in Japan. The genes responsible for autosomal dominant albinism have not been identified in any animal species. After confirming that this trait is not due to a tyrosinase gene mutation that causes OCA1 (tyrosinase-negative OCA) using a tyrosinase intragenic microsatellite marker, the dominant albino locus has been mapped on linkage group G of the reference linkage map of rainbow trout. To improve mapping resolution, 671 individuals obtained from a cross between a heterozygote (genotype A/a for dominant albino) and normal type (a/a) were used for linkage analysis. A detailed linkage map of which the genetic distances between the dominant albino locus and markers were within the range of 0.6-2.2 cM was constructed. We isolated 172,032 BAC clones from one of the previously constructed rainbow trout BAC library and individually deposited in micro titer plates (384-well/plate), and made high-density replica (HDR) filters from them. It would facilitate construction of physical map for positional cloning of the dominant albino gene and contribute better understanding of tyrosinase-negative OCA in animals.