Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Workshop: ITMI
            

SEQUENCE INFORMATION INDEPENDENT TECHNOLOGIES FOR GENOMICS

¹ CAMBIA, Clunies Ross Street, Canberra, ACT 2601, Australia

Two technologies developed by CAMBIA's genomics program will be presented. One is Transgenomics Initiative, the program that concentrates on developing novel traits and agriculturally relevant characteristics. TGI develops genetic materials that will help with our understanding of genetic networks in plants. The controlled manipulation of expression of practically any gene in rice offers an opportunity to develop and test specific hypotheses of linkages between gene expression and the resulting phenotype. Current stage of Rice TGI will be reported, with main emphasis on development of comprehensive population of Transcriptional Activator Facilitated Enhancer Trap (TAFET) lines. Complementary to novel trait development CAMBIA is developing new approaches to high throughput genetic analysis using DNA microarrays. The main technology, Diversity Arrays (or DArT) offers significantly higher sample throughput, lower cost as well as minimal DNA sample requirement compared to other technologies. CAMBIA's approach will offer simultaneous genotyping and pathogen diagnostics/detection on a single slide. Development of Diversity Arrays and the application of DArTs to germplasm characterization and mapping will be presented.