¹ Genome Research Center. KRIBB. P. O. Box 115, Yusong, Taejon, Republic of Korea

Large scale single-pass sequencing of cDNA has proven to be a useful tool for discovery of new genes and understanding of biological mechanisms. As a first step to understand the complexity of plant defense mechanism, expressed sequence tags (EST) were generated from hot pepper leaf cDNA library constructed from combined leaves prepared at different time after inoculation with soybean pustle pathogen (Xanthomonas campestris pv glycines). For future extension of gene diversity, ESTs were also generated from cDNA libraries constructed fron anther, and flower bud using dye termination method. Among total of 10,161 generated ESTs, 9434 had good quality to be anayzed and clustering analysis revealed that 50% of total ESTs (4685) were unique. Here we present detailed sequence analysis data. This is the first work of the massive sequence information about hot pepper plant (Capsicum annuum L.).