Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Poster: Genome Sequencing & ESTs
The NSF-funded Wheat Genome Project is isolating a large number of expressed sequence tags (ESTs). Detailed investigations of the structure and function of expressed sequences dictate special approaches in wheat because of its large and complex genome. Wheat aneuploid stocks including 21 Nullisomic-Tetrasomic (NT), 24 ditelosomic (Dt), and 101 deletion (del) lines were selected to allocate ESTs to specific chromosome bins. The objective of this study is to provide detailed molecular characterization of these deletion stocks. The 387 EST probes were used to screen the NT, Dt, and deletion lines. A total of 1406 loci were detected by 364 informative EST probes and assigned to 21 chromosome arms spanning regions from the centromere to the telomere. The previous described deletion lines del1AS-4, del6AL-2, del6BS-6, and del7DS-6 were found to have normal chromosome constitution. Del3AS-3 is nullisomic for the entire 3AS arm; the deficient segment of the short arm may be a translocation from an unknown chromosome. New deletions were detected in twenty-six deletion lines. Twenty lines had one new deletion, four lines had two, one line had three, and one line had four new deletions. A few deletions were interstitial. However, most of the new deletions occurred in terminal regions of chromosomes, and probably resulted from the loss of very small terminal fragments difficult to detect by cytological analysis. In addition, new chromosome aberrations in NT and Dt lines were also identified.