Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Poster: Gene Isolation
            

ISOLATION OF cDNAS INVOLVED IN THE EPISTATIC INTERACTION BETWEEN HOODED (KAP) AND SHORT-AWNED (LKS2) IN THE OREGON WOLFE BARLEYS

¹ Dept. of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA

The Oregon Wolfe Barleys (OWB) are a population of 94 doubled haploids (DH) derived from the F₁ of a cross between Dr. R. Wolfe's dominant and recessive marker stocks. Eleven easily-scored morphological traits that are segregating in this population were positioned on a comprehensive linkage map built with an array of molecular markers. In individuals that are homozygous for the recessive allele of lks2, the hooded (Kap) phenotype is masked, resulting in a short-awned, non-hooded inflorescence rather than the hooded phenotype. This epistatic interaction precluded the mapping of the hooded trait. Instead, a molecular marker, Hvknox3, was used to locate Kap in the linkage map. Restriction Fragment Differential Display in combination with Bulk Segregant Analysis was used to isolate cDNAs involved in the epistatic interaction between lks2 and Kap. The OWB DH population was divided into four bulks, based upon the genetic makeup at the Kap and lks2 loci. Floral tissue for RNA extraction was collected from individuals of each bulk and the OWB parental lines at the terminal period of the elongating lemma primordia stage. From the comparison of the patterns of gene expression in each of the four bulks we expect to identify cDNAs linked to Kap and lks2, as well as cDNAs that are involved in the epistatic interaction between the two genes.