Agricultural Microbes Genome 2 Conference

Workshop: CSREES Grant Awardees
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Phytophthora sojae and Phytophthora infestans

¹ University of California, Davis, CA

² University of California, Riverside, CA

³ North Carolina State University

⁴ National Center for Genome Resources

ORGANISM STRAIN:
Phytophthora sojae and Phytophthora infestans

BRIEF SUMMARY OF SEQUENCING STRATEGIES:
We will sequence cDNAs (ESTs) from various axenic and infection tissues. Post-cloning subtraction strategies will be used to enrich for new sequences. Subtraction and positive selection will be used to enrich for pathogen sequences in infection libraries. EST clones will be matched to a BAC library by a novel PCR-based matching procedure called BEST-MAP.

DATA RELEASE PLAN:

• Website Address(es): www.ncgr.org/pgi
• Frequency of Data Release & Updates: Beginning February 2001, new EST sequences will be released onto the website as they are generated, via an automated sequence processing pipeline

TIMELINE FOR COMPLETION:

• Start date: 9/15/00
• B. Anticipated completion date: 9/30/02
• C. Milestones:
  • 1/31/01 new version of website operational, new sequences begin appearing;
  • 6/30/01 24,000 new EST sequences;
  • 12/31/01 40,000 new ESTs;
  • 12/31/01 new generation database and website operational;
  • 12/31/01 EST BAC mapping begins;
  • 6/30/02 55,000 ESTs;
  • 9/30/00 EST BAC mapping complete.

PLAN FOR MAINTENANCE & DISTRIBUTION OF REAGENTS:
EST clones stored at originating labs and a duplicate at NCState. P. sojae clones will be distributed form UC Davis. P. infestans EST clones will be distributed from UC Riverside. Supplier labs will pay for preparation of materials. Requestors will pay shipping.

PROJECT CONTACT:
Name: Brett Tyler Phone: 530-752-4771 Fax: 530-752-5674 Email: bmtyler@ucdavis.edu