Agricultural Microbes Genome 2 Conference

Workshop: CSREES Grant Awardees
W05_02.html

Mycobacterium avium subspecies paratuberculosis, strain K-10

¹ University of Minnesota, St. Paul, MN

² National Animal Disease Center, Ames, IA

³ Michigan State University, East Lansing, MN

ORGANISM STRAIN:
Mycobacterium avium subspecies paratuberculosis, strain K-10

BRIEF SUMMARY OF SEQUENCING STRATEGIES:
A random shotgun library was prepared of the ~ 6Mb genome in plasmid vectors and ~ 60,000 sequencing reactions performed. The individual sequence fragments have been assembled with phredPhrap and Consed. The contigs are being assembled through a combination of primer walking and PCR-based approaches.

DATA RELEASE PLAN:

• A. Website Address(es): www.cbc.umn.edu/ResearchProjects/AGAC/Mptb/Mptbhome.html
• B. Frequency of Data Release & Updates: At least bi-weekly or after 3000 additional reads have been generated and assembled.

• A. Start date: September, 2000
• B. Anticipated completion date: December, 2001 for final sequence assembly and annotation
• C. Milestones:
  • Library Preparation and validation: Sept/Oct of 2000
  • Completion of random shotgun phase 60k reads: December, 2000
  • Final Assembly, June 2001
  • Annotation of sequence: December, 2001

PROJECT CONTACT:
Name: Vivek Kapur Phone: 612-625-7712 Fax: 612-625-5203 Email: vkapur@umn.edu