Agricultural Microbes Genome 2 Conference

Workshop: CSREES Grant Awardees
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Fibrobacter succinogenes strain S85 / Ruminococcus albus strain 8

¹ The Ohio State University

² The Institute for Genomic Research

³ Cornell University

⁴ University of Guelph, Canada

⁵ The University of Illinois

ORGANISM/STRAIN:
Fibrobacter succinogenes strain S85 (to closure) and Ruminococcus albus strain 8 (to 8x genome coverage)

BRIEF SUMMARY OF SEQUENCING STRATEGIES:
We propose to sequence the genomes of these bacteria using the random shotgun strategy and vectors routinely used by TIGR. This approach will enable us to ultimately obtain the complete genome sequence, including gap closure, finishing, and annotation, for each genome. The methods have been successfully used for all of TIGR's recent microbial genome projects, effectively accommodating variations in genome size (560 kb to 6.2 Mb), base compositions ranging from 19% to 67% G+C, and the presence of various repeat elements, IS elements, and multiple chromosomes and plasmids.

DATA RELEASE PLAN:
Website Address(es): www.tigr.org

Frequency of Data Release & Updates:
The genome sequence data for this project will be released within one month of reaching 3-fold coverage for each bacterium, and monthly thereafter until the projects reach completion. Release will be limited to contigs of 1 kb or greater. The relational databases that result from annotation are used to run the TIGR World Wide Web site (www.tigr.org) and a specific website will be created for the Rumen Microbial Genomics program at TIGR. These databases will provide the complete DNA sequence for both bacteria and the translation of all ORFs on an ftp site, as well as gene identifications and alignments, and a searchable list of all genes and their metabolic role categories. In addition, the contigs will be searchable through the TIGR Blast Search Engine for Unfinished Genomes (www.tigr.org). Investigators subscribing to this service (free of charge to academic investigators) receive notification of all new releases of sequence data.

TIMELINE FOR COMPLETION:

• Start date: January 1st 2001
• B. Anticipated completion date: December 1st 2001
• C. Milestones: Sequencing of both genomes should be completed by the end of 2001

PLAN FOR MAINTENANCE & DISTRIBUTION OF REAGENTS:
The various libraries will either be maintained at The Ohio State University and(or) a suitable private, vendor such as the Amercian Type Culture Collection. A nominal charge to cover the costs of maintaining the collection and mailing, etc.,. will be imposed.