Agricultural Microbes Genome 2 Conference Agricultural Microbes Genome 2 Conference
Poster: Genetic, Mutational, and Biochemical Technology
P06_01.html
A novel method to purify PCR products from polymerase chain reaction contaminants has been developed using MagneSil™, Promega’s proprietary paramagnetic silica particles (PMPs). MagneSil™ PMPs serve as a “mobile solid phase” to purify PCR products in a high-throughput, automated manner, at an affordable cost for high-throughput screening (HTS) labs. Paramagnetic particles offer a flexibility that is not seen with centrifugation and vacuum-based formats for nucleic acid purification. Among these benefits are scalability, easier handling and in-solution kinetics. Many applications downstream of PCR require purification of PCR products away from unincorporated primers, primer-dimers, and other reaction components. Existing methods for purifying PCR products are cumbersome, difficult to automate and time consuming. The automated MagneSil™ system can be used to purify 96 PCR reactions in approximately 1.5 hours, using a Tecan Genesis RSP 150 robotic workstation. By optimizing the binding and wash conditions, PCR products larger than 150bp are selectively purified for use in downstream applications, such as microarraying, DNA sequencing, cloning and restriction digestion.