Agricultural Microbes Genome 2 Conference Agricultural Microbes Genome 2 Conference
Poster: Genome Technology
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A Mycoplasma hyopneumoniae subunit based vaccine is of considerable value to the pork industry. We have applied a number of antigen discovery technologies to this bacteria, one being Rational Antigen Choice (RAC) in which genes encoding antigens are chosen on theoretical considerations pertaining to their sequence. To date we have sequenced genomic library clones representing approximately a third of the M. hyopneumoniae genome, and in conjunction with data from two completed mycoplasma genomes, have targeted groups of clones that we consider potential antigens for trial in vaccine / challenge experiments. RAC results have provided useful leads towards the development of a subunit vaccine, however, these are based on genomic library clones containing primarily partial gene sequences. Therefore, we have developed our own macro-arrayed library and screening procedure to rapidly isolate the complete sequence of a large number of the RAC genes of interest. The development of our library macro-arrays, their use and adaptation to other array techniques will be presented.