Agricultural Microbes Genome 2 Conference

Poster: Genome Technology
P04_01.html

AUTOMATION OF DNA PURIFICATION FROM PLANT MATERIALS USING MAGNESIL PARAMAGNETIC PARTICLES

¹ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711

² Address:

MagneSil paramagnetic particles allow the flexibility of automating the isolation of DNA from as little as 20mg of plant material to as much as 500 grams of vegetable oil for use in nutritional or authenticity testing , or plant breeding applications such as random amplification polymorphism detection (RAPD) amplified fragment length polymorphism, (AFLP) or polymerase chain reaction (PCR). Given the wide variety of plant materials that require testing, the purification system must be both scalable and flexible in its ability to purify DNA from such a wide variety of sample types. The procedures used in these purification systems are similar to other methods used for Promega's walkaway automation of plasmid purification and DNA sequencing reaction cleanup used in genomics applications, as well as DNA purification of DNA from PCR reactions used for genetic interogations or DNA immobilizations. These MagneSil based purification systems can be used with a variety of robotic workstations in 96 well formats.