Plant & Animal Genome IX Conference

Workshop: Plant Cytogenetics
W46_07.html

CREATION OF A WHEAT-THINOPYRUM INTERMEDIUM HOMOGENOUS TRANSLOCATION LINE BY ANTHER CULTURE OF MONOSOMIC ADDITION LINES

¹ Beijing Agriculture Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, Banjing cun, Haidian district, Beijing, 100089, P. R. China

² Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Baishiqiao Road 30, Beijing 100081, P. R. China

It is very important to transfer useful genes from wild relatives of the tribe Triticeae to wheat. The wheat-Thinopyrum intermedium homogenous translocation line was firstly successfully produced by a cell bio-engineering method, anther culture of monosomic addition lines. The translocation line 99-803 was identified from pollen-derived plants of F1 (LA868^9-XXX-2,Wheat accessions í L1,Th. intermedium disomic addition line) among 7 combinations, 20 pollen-derived plants. The results of cytological and GISH analysis indicated that meiotic chromosome configuration of PMCs of the translocation line was 18.52 ring bivalents + 2.57 rod bivalents + 0.01 univalents. The translocation segment from Th. intermedium was very short. Among 102 PMCs observed, the GISH signals in 89.2% of PMCs were located in one of cross points of ring bivalents, 9.8% in the middle crosses of rod bivalents, only 1% in the poles of rod bivalents. Non-robertsonian translocation was produced between unidentified chromosome from wheat and 7Ai-1 from Th. intermedium. The frequency of the got translocation line was more than 2%. The results indicate that this procedure is an effective and expeditious way for transferring alien chromosome segments (genes ) from other species to wheat.