Plant & Animal Genome IX Conference

Poster: Forest Trees
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DEVELOPMENT OF CAPS MARKERS IN Chamaecyparis obtusa BASED ON ESTs FROM Cryptomeria japonica

Forestry and Forest Products Research Institute, 1 Matsunosato, Kukizaki, Ibaraki 305-8687 Japan

Sugi (Cryptomeria japonica) is one of the most important conifers in Japanese forestry. We have obtained nearly four thousand partial sequences of ESTs (expressed sequence tags) from sugi(Cryptomeria japonica) cDNA library and the Genome Database is now available. A large number of useful STS markers (474 STSs) for sugi genome mapping and population study have been developed using those information until now. In this study, we applied sugi STS primers to hinoki (Chamaecyparis obtusa ) which is closely related conifer to sugi. In the result of PCR amplification, 122 out of 474 STS primers gave single fragments in hinoki genome. Those fragments were simultaneously sequenced and confirmed the homologies to original sequences of cDNA clones. To develop CAPS (cleaved amplified polymorphic sequences) markers, 16 hinoki plus trees including the parents of mapping population were amplified and digested with 28 restriction enzymes. Polymorphisms of STS fragments were detected and eventually 51 CAPS markers were successfully developed. Segregation pattern was also verified using mapping population. The result indicated that approximately 26% of STS markers derived from sugi ESTs can be utilized as hinoki STS markers and almost half of markers were polymorphic. We will construct a hinoki linkage map using these CAPS markers as well as other marker systems in future work.