Plant & Animal Genome IX Conference

Poster: Large Insert Libraries, Gene Isolation, Etc.
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DEVELOPMENT OF HIGH COPY BAC VECTOR (pCUGIBAC2) AND CONSTRUCTION OF TWO BAC LIBRARIES

¹ National Institute of Agricultural Sciences and Technology, 249 Serdundong, Suwon, Kyunggido, 441-707, Korea

² Clemson University Genomics Institute, 100 Jordan Hall, Clemson University, Clemson, South Carolina, 29634, USA

In order to increase the efficiency of BAC vector DNA preparation, we develop a high copy number of pBACwich (pCUGIBAC2), capable of integrating large DNA fragment into plants using the cre/lox site specific recombination system. The new vector was used for construction of two BAC libraries. One library which is from an inbred line of Chinese cabbage (Brassica campestris L. ssp. pekinensis), one of the parents of the hybrid Jangwon used in a permanant mapping population maintained by the NIAST cabbage genome program and the second library is from native korean cultivar of Red pepper (Capsicum annuum cv. subicho). In the case of the Chinese cabbage BAC library, the total number of obtained clones is 73,728 and provides 12.5X genome equivalents. The library is contained in four high density filters (18,432 clones per filter). The average insert size was estimated as 140Kb. The Red pepper BAC library consists of 184,320 clones and provides 5X genome equivalents. The average insert size was estimated as 140Kb. The library is contained in ten high density filters. Filters from both libraries are now available.