Plant & Animal Genome IX Conference

Poster: Large Insert Libraries, Gene Isolation, Etc.
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BAC LIBRARY CONSTRUCTION AND MAPPING USING RESISTANT GENE ANALOG POLYMORPHISM (RGAP) IN CHICKPEA

¹ USDA-ARS and Dept.of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434

² Dept. of Plant Soil and General Agriculture, Carbondale, IL 62901-4415

Chickpea is the third most important pulse crop in the world after dry beans and dry peas and first in India and Mediterranean region. Though the area of chickpea cultivation is large, productivity is low because of susceptibilty to diseases. Among them, ascochyta blight and fusarium wilt cause major damage to chickpea production. Genes for resistance to these diseases have been mapped in the chickpea genome. Our present goal is to isolate the disease resistance genes using a large insert Bacterial Artificial Chromosome (BAC) library using information on genetic linkage with the markers and also enrich the map with Resistant Gene Analog (RGA) markers. With this objective,we constructed a BAC library from FLIP 84-92C which was the source of resistance to ascochyta blight.The average insert size is 120kb.The library comprises 23680 colonies which corresponds to 3.8 times of the genome.This BAC library will be probed with markers already mapped and closely linked to ascochyta blight resiatnce genes.This is the first Chickpea BAC library constructed so far.Bulk Segregant Analysis(BSA) was performed with RGA primers and the polymorphic primers were run in the population to map resistant loci.Mapping RGA markers will be useful to find closely linked markers to the blight resistant genes which would ultimately help to identify the resistant gene from BAC library.If successful,we would determine the sequence of the isolated genes and possibly develop a direct marker for use in marker assisted selection.