Plant & Animal Genome IX Conference

Poster: Large Insert Libraries, Gene Isolation, Etc.
P02_10.html

CHARACTERIZATION OF THE CENTROMERIC REGION OF A PLANT MINICHROMOSOME

Plant Molecular Cytogenetics Group, Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany

The chromosome mutant PRO1 contains a single wild beet chromosome fragment (minichromosome) of the wild beet Beta procumbens in a sugar beet (Beta vulgaris) background. As the minichromosome is faithfully inherited in mitosis and species-specific, centromeric probes are available this mutant provides a suitable system for the analysis of an individual plant centromere. Using wild beet-specific centromeric satellite repeats we screened a PRO1 BAC library. Positives BAC clones were used as probes in fluorescent in situ hybridization experiments to PRO1 and B. procumbens chromosomes to reveal their wild beet origin and their centromeric localization. A contig of BAC clones spanning the centromeric region of the minichromosome will be established and characterized on a molecular level. This work is founded by grants from the BMBF (BioFuture grant 0311860) and DFG (Schm1048/2-3).