Plant & Animal Genome IX Conference

Poster: Large Insert Libraries, Gene Isolation, Etc.
P02_08.html

A BAC/EST LIBRARY RESOURCE FOR CROP GENOMICS

¹ Clemson University Genomics Institute, 100 Jordan Hall, Clemson SC 29634

² Fungal Genomics Laboratory, Plant Pathology, NC State University

Clemson University has established a Genomics Institute (CUGI) focusing on research, teaching and service in agricultural genomics. One service component of CUGI is to produce and distribute high quality BAC and EST libraries from plants and plant pests and to provide convenient and affordable access to these libraries, high-density hybridization filters, clones and related technologies. With the assistance of several high-throughput robotic devices, CUGI has produced, acquired and distributed the majority of plant and fungal BAC libraries in use today. Over the past year, we have constructed a number of libraries and presently maintain in excess of 5 million clones. We currently have 59 deep coverage libraries from plants, bacteria and fungi available for distribution. CUGI is divided into five Centers: 1) BAC/EST Resource Center; 2) Physical Mapping Center; 3) DNA Sequencing Center; 4) Bioinformatics Center, and 5) Functional Genomics Center. The services provided by CUGI are: 1)Picking, arraying and rearraying of BAC and EST clones 2)Distribution of BAC and EST clones 3)BAC library screening by hybridization 4)BAC end sequencing and shotgun sequencing of entire BACÆs and BAC contigs 5)Fingerprinting of BAC clones identified by hybridization or PCR 6)Develop microarray technology for gene expression profiling 7)Provide and maintain a centralized database for BAC fingerprints, hybridization and BAC end sequence data 8)Provide training and assistance in BAC library construction and clone manipulation We will present results from our most recent experiments in library construction.