Plant & Animal Genome IX Conference

Poster: Sequencing & EST
P01_82.html

ENDOSPERM ESTs FROM Triticum aestivum CV. CHEYENNE

USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA, 94710, USA

We have generated 4916 Expressed Sequence Tags (ESTs) from the cDNA library of the endosperm of Triticum aestivum cv. Cheyenne, a cultivar that gives high quality flour for bread baking. Using a clustering program to assemble the genes, the ESTs grouped into 594 tentative contigs (TC) and 1873 tentative singletons (TS), identifying 2467 tentative unique genes. BLAST similarity search against NCBI non-redundant sequence database revealed abundant messages for storage proteins (i.e. ~10% of the ESTs are gliadins) and proteins involved in starch metabolism. Approximately 27% of the ESTs showed homology to genomic DNA sequences of unknown function. Comparison of the endosperm ESTs from the ESTs generated from other tissues of the wheat plant showed low TS and TC overlap between tissues.