Plant & Animal Genome IX Conference

Poster: Sequencing & EST
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GENOME WIDE APPROACH TO UNDERSTANDING OF NON-HOST RESISTANT IN PEPPER PLANTS

¹ Plant Cell Biotechnology Lab, Korea Research Institute of Bioscience and Biotechnology, Yusong P.O. Box 115, Taejon 305-600, Korea

² Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong P.O. Box 115, Taejon 305-600, Korea

Identification of a complete set of genes involved in the defense process is an essential step toward understanding of whole scheme of plant defense mechanisms. We are pursuing to isolate all of the hot pepper genes that are expressed during plant-pathogen interaction. To contribute to this goal, a cDNA library was constructed from pepper (Capsicum annuum) leaves showing non-host hypersensitive response against bean pustule pathogen (Xanthomonas campestris pv. glycines). Around 2500 expressed sequence tags (ESTs) were generated and analyzed for redundancy within the cDNA library. About 50% of the ESTs were independent genes. The sequences of ESTs were searched against available database(s) through the NCBI using the BLASTX program and classified according to putative function based on similarity to known proteins. In conclusion, ESTs were grouped into eight functional categories; photosynthesis/metabolism, signal transduction, disease/defense, secondary metabolism, transporter, structure/protein synthesis, hypothetical protein, and unknown. The unique EST clones will be arrayed on a slide glass and transcription profiles following pathogen infection will be analyzed. In addition, more ESTs will be sequenced and analyzed.