Plant & Animal Genome IX Conference

Poster: Sequencing & EST
P01_65.html

ANALYSIS OF EXPRESSED SEQUENCE TAGS OF FLOWER BUDS IN PHALAENOPSIS EQUESTRIS

¹ Department of Biology, National Cheng Kung University, Tainan 701, Taiwan

² Institute of Biotechnology, Department of Biology, National Cheng Kung University, Tainan 701, Taiwan

³ Department of Horticulture, Taiwan Sugar Research Institute, Tainan 702, Taiwan

For comprehensive analysis of genes expressed in a reproductive organ of Phalaenopsis, we constructed a cDAN library of mature flower buds of P. equestris, and characterized randomly selected expressed sequence tags (ESTs) of 781 clones. From BLASTX search analysis of GenPept, PIR and SwissProt protein databases, 45.2% of the ESTs showed significant sequence similarities to known genes. Among the 781 sequences, 161 (20.6%) showed strong similarity to metabolic enzymes, 54 (6.9%) have homology with proteins which are involved in protein synthesis, 20 (2.6%) are functional related to RNA transcription process, 32 (4.1%) are similar to stress or defense-induced proteins, 27 (3.5%) are related to signal trsansduction pathway and 8 (1%) are involved in floral development. The EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of P. equestris.