PAG-IX: SEQUENCING AND CHARACTERISATION OF ADIPOSE, SKELETAL MUSCLE, HYPOTHALAMUS, ABOMASUM AND DUODENUM cDNA LIBRARIES IN Bos taurus CATTLE.

PAG-IX   Plant & Animal Genome IX Conference

Town & Country Hotel, San Diego, CA, January 13-17, 2001.

Poster: Sequencing & EST
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SEQUENCING AND CHARACTERISATION OF ADIPOSE, SKELETAL MUSCLE, HYPOTHALAMUS, ABOMASUM AND DUODENUM cDNA LIBRARIES IN Bos taurus CATTLE.

STEPHEN S MOORE, Chang Li, Anna Fu, Yan Meng, Gordon Murdoch, Robert Christopherson, Walter Dixon

Department of Agricultureal, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5

Identification of genes responsible for net feed efficiency has great commercial potential in the beef industry. Feed accounts for over 50% of the total production costs for beef in Canada. To facilitate a comprehensive gene discovery program aimed at identifying genes important in feed intake and efficiency, cDNA libraries for adipose, skeletal muscle, hypothalamus, abomasum and duodenum of Bos taurus cattle were constructed. About one thousand ESTs randomly picked and sequenced from each of the cDNA libraries are being analysed. The distribution of EST sizes and the rate of the redundancy before and after the normalization of the libraries are examined and reported for the muscle library. Further large scale sequencing results from all tissues will contribute to the growing EST resource for cattle.

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