PAG-IX: ANALYSIS OF WOOD DEVELOPMENT WITH A GENOMIC APPROACH: EUCALYPTUS ESTS AND TAC GENOMIC LIBRARY

PAG-IX   Plant & Animal Genome IX Conference

Town & Country Hotel, San Diego, CA, January 13-17, 2001.

Poster: Sequencing & EST
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ANALYSIS OF WOOD DEVELOPMENT WITH A GENOMIC APPROACH: EUCALYPTUS ESTS AND TAC GENOMIC LIBRARY

SHIGERU SATO, Keiko Horikiri, Kyoko Miyashita, Naoko Ishige, TAKASHI HIBINO, Tetsu Kawazu

24-9 Nobobocho, Kameyama, Mie, 519-0212, Japan

A forest tree is one of the valuable biomass resources in industry. Understanding the physiology of wood development is important for utilization of the biomass. To get comprehensive understanding the development, we are collecting the genome-scale information in forest trees. ESTs are being obtained from differentiating xylem and leaves of several Eucalyptus species that are used as model trees. Purposes of this project are 1; to identify a large number of ESTs, 2; to analyze their expression profiles using cDNA arrays, 3; to compare Eucalyptus ESTs with other plant ESTs. On the basis of the genome-scale information, we want to focus on gene expression peculiar to forest trees. We are also constructing TAC (Transformation-competent Artificial Chromosome) genomic libraries in several Eucalyptus species. The current status of this project will be discussed.

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