Plant & Animal Genome IX Conference

Poster: Sequencing & EST
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ISOLATION OF DEFENSE RESPONSE GENES FROM RICE USING SUPPRESSION SUBTRACTION HYBRIDIZATION

Dept. of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502,USA

Using suppression subtractive hybridization, a cDNA library has been constructed for rice to identify genes that are differentially expressed during incompatible and compatible interactions with the bacterial blight pathogen, Xanthomonas oryzae pv. orzyae. Leaves of rice cultivar IRBB10 carrying the Xa10 gene for bacterial blight resistance were harvested for RNA extraction at 0, 4, 8, and 12 h after infiltration with a strain of X. oryzae pv. oryzae with avirulence gene avrXa10. After subtractive hybridization and cloning, non-redundant cDNA clones were selected and sequenced. Based on their putative function, 78 non-redundant clones were classified into the following groups: 12 known defense/stress response genes (e.g., rice inducible peroxidase), 5 signal pathway genes (e.g., putative protein kinase), 7 DNA interacting/transcription factor genes (e.g., Myb-related protein), 7 rice EST's of unknown function, and 20 with no match in the databases. Northern blot analyses using a subset of the clones as probes are underway to confirm the differential expression of the genes during defense responses.