PAG-IX: CONSTRUCTION OF A WHEAT CDNA SUBTRACTION EST LIBRARY FOR KARNAL BUNT DISEASE RESISTANCE ANALYSIS

PAG-IX   Plant & Animal Genome IX Conference

Town & Country Hotel, San Diego, CA, January 13-17, 2001.

Poster: Sequencing & EST
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CONSTRUCTION OF A WHEAT CDNA SUBTRACTION EST LIBRARY FOR KARNAL BUNT DISEASE RESISTANCE ANALYSIS

Kristi L. Hill-Ambroz, JOHN P. FELLERS, Gina L. Brown-Guedira

USDA-ARS, 4008 Throckmorton Hall, Kansas State University, Manhattan, KS 66506

Karnal Bunt (KB) of wheat originated in Karnal, India and causes substantial yield and quality losses. KB has been regarded as severe threat to the United States wheat industry and the United States Department of Agriculture and has led to strict quarantine regulations on the movement of potentially infected KB wheat into the United States and other KB-free countries. Resistant (HD29) and Susceptible (WL711) Indian spring wheat cultivars were used to construct a subtracted cDNA library enriched for the resistant genotype. mRNA was isolated from non-infected wheat spikes in the boot stage of development. 960 ESTĘs have been isolated, sequenced and subjected to BLASTX searches. There were 257 ESTs over 500 bp in size. To date, 3 clones have similar alignments to potential resistant genes. Also, multiple sequences aligned with hypothetical or unknown protein sequences and have the potential to be new genes associated with KB resistance. The 257 larger clones will be further analyzed for their relationship to KB resistance and this data will be reported.

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