Plant & Animal Genome IX Conference

Poster: Sequencing & EST
P01_37.html

A LARGE SCALE BARLEY cDNA SEQUENCING PROGRAM IN OKAYAMA UNIVERSITY

¹ Barley Germplasm Center, Research Institute for Bioresources, Okayama Univ., Kurashiki, 710-0046, Japan

² Akita Prefectural Univ., Akita, 010-0146 Japan

We have a program of sequencing a total of ca. 30,000 barley cDNA clones in Okayama University by the cooperative project with National Institute of Genetics in Japan. A set of cDNA libraries have developed from the various stages and organs in the genotypes Haruna-Nijo (Japanese high quality malting barley), Akashinriki (Japanese naked landrace) and Hordeum vulgare ssp. spontaneum (H602). The clones have been sequenced from both 3' and 5' ends and searched by the BLAST to estimate the function. A preliminary sequence comparison between cDNA libraries showed that the redundancy of the clones was relatively low among genotypes. Our main interest is to use cDNA analysis to catalogue barley genes and to characterize barley genotypes preserved at Barley Germplasm Center in Okayama University. Genetic stocks such as mutants, linkage testers, isogenic pairs may be the appropriate materials for the gene discovery. Currently we are maintaining up to 10,000 barley accessions in Okayama University mainly local landraces from the world. The cDNA based fingerprinting will be used to estimate the genetic composition of the accessions, to estimate the evolutional process of cultivated barley and to modify the germplasm collection preserved in the genebank..