PAG-IX: GENOMIC STRUCTURE OF THE LOCUS AROUND JOINING SEGMENTS OF PORCINE T CELL RECEPTOR ALPHA AND ITS COMPARATIVE ANALYSIS AGAINST THE LOCI OF HUMANS AND MICE

PAG-IX   Plant & Animal Genome IX Conference

Town & Country Hotel, San Diego, CA, January 13-17, 2001.

Poster: Sequencing & EST
P68

GENOMIC STRUCTURE OF THE LOCUS AROUND JOINING SEGMENTS OF PORCINE T CELL RECEPTOR ALPHA AND ITS COMPARATIVE ANALYSIS AGAINST THE LOCI OF HUMANS AND MICE

HIROHIDE UENISHI1, Hideki Hiraiwa2, Takeshi Hayashi1, Ai Hoshikawa1, Yohtaroh Takagaki3, Takashi Shiina4, Eri Kikkawa4, Hidetoshi Inoko4, Takashi Awata1, Hiroshi Yasue1

1 Department of Animal Breeding and Genetics, National Institute of Animal Industry, Norindanchi P.O.Box 5, Tsukuba, Ibaraki 305-0901, Japan
2 Second Research Division, STAFF-Institute, Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0901, Japan
3 Department of Molecular Biology, Kitasato University School of Medicine, Kitasato, Sagamihara, Kanagawa 228-8555, Japan
4 Department of Molecular Life Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan

T cell receptor (TCR) delta gene is located between the region encoding variable and constant region of TCR alpha chain. This region of humans and mice has been sequenced to completion and it was revealed that this region is well conserved between these two species. However, it remains unclear whether this region is conserved in other mammalian species. Here we report the genomic sequence encompassing constant regions of porcine TCR alpha and delta gene, and J alpha segments between the alpha and delta constant regions. We have determined about 132 kb of porcine genomic sequence including the constant regions of TCR alpha/delta. Four TCR J alpha segments are identified in the upstream region of TCR C delta. The sequence between C alpha and C delta was demonstrated to contain more than fifty J alpha segments, some of which harbored pseudogenes, spanning 80 kb. One V delta segment is located between C delta and J alpha in the reverse direction. This region also includes the T early alpha (TEA) element. Moreover, in the downstream of the C alpha, there are a TCR alpha enhancer element and DAD1 gene. Mammalian-wide interspersed repeats (MIRs) are contained more than other genomic region, such as SLA, and distribution of the MIRs is well conserved among pigs, humans and mice. This implies that the clustering structure of J alpha was constructed at the stage of their common ancestor.

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